DMEM/F-12 (1:1 mix) (with L-glut and sodium pyruvate)

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium that can support the growth of many different types of mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation contains the high concentrations of glucose, amino acids, and vitamins from DMEM and various components from F-12. DMEM/F-12 does not contain proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require nutrient supplementation, typically with 10% fetal bovine serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffering system and therefore requires a 5% - 10% CO2 environment to maintain physiological pH.

Storage Conditions: Store at 2-8℃ away from light

Transportation Conditions: Room temperature

Shelf Life: 12 months from the date of manufacture